Saturday, January 25, 2020

Fatty Acid Profile Oil Contents Tocopherol on pigeon

Fatty Acid Profile Oil Contents Tocopherol on pigeon Pigeons, whose relation with humans traces back to ancient times (B.C. 3000-5000), are seen in almost every geographical region of the world except for the poles. Pigeons live side by side with humans and other animal species in the nature; and they are bred as a source of food, as a hobby, symbol, and for experimental aims (cooper et al.,1984). Pigeons constitute the bird family Columbidae within the order columbiformes (Baptista et al.,1997).All races of breeds of domestic pigeon Columba livia domestica have been evolved from rock pigeon also called a rock dove Columba livia livia or jungli kabootar (wild pigeon) that cohabits with man every where in the world. The domestic pigeon breeds are believed to have been developed in the east and Europe had no original breed of its own. Wild pigeons are pale grey with two black bars on each wing (Blechman et al., 2007). There has been a growing interest in meat from alternative animal species like deer (Volpelli et al., 2003),ostriches (Cooper, 1999; Horbanczuk, 2002), and pigeons (ZieleziÅ„ski and Pawlina, 2005). Lean meat is very low in fat (20-50g/kg), pork and poultry have a favourable balance between polyunsaturated and saturated fatty acids (P : S) (Wood et al., 1997 ). Pigeon meat is considered a delicacy and is gaining popularity among consumers in Europe mainly in Great Britain, France, and Italy as well as in the United States and China (ZieleziÅ„ski and Pawlina, 2005). Pigeon meat is characterized by high nutritive value. Due to low cholesterol and a fairly high protein content, it can be used as a valuable inclusive component of the human diet. As far as FA composition is concerned, pigeon meat resembles meat types of poultry species (Pomianowski et al ., 2009). Vitamin E is a general term used for the designation of tocopherols and tocotrienols (ÃŽÂ ±-,ß-, ÃŽÂ ³-, ÃŽÂ ´-) (Acker et al ., 1993) . Variations in vitamin E deposition in fatty tissues have been found between different poultry species (Mecchi et al., 1953). It was found that concentrations of tocopherol in turkey liver and breast muscle were only one-fifth to one-third, respectively, those of broilers fed similar dietary levels (Marusich et al., 1975). They function as the most effective lipid soluble antioxidant ,protecting cell membranes from peroxyl radicals and mutagenic nitrogen oxide species (Acker et al ., 1993). Tocopherols provide immunoprotection (Meydani ,1995), antiproliferative (Azzi et al .,1995) and anticlotting effects (Dowd et al .,1995),platelet adhesives and thrombosis (Hodis et al ., 1995) . Increased vitamin E intake has been inversely associated with the lower risk of cardiovascular and coronary heart diseases (Losonczy et al.,1996). Bells palsy or idiopathic facial paralysis is a dysfunction of cranial nerve VII (the facial nerve) that results in inability to control facial muscles on the affected side. Several conditions can cause a facial paralysis, e.g., brain tumor, stroke, and Lyme disease. However, if no specific cause can be identified, the condition is known as Bells palsy(Salinas et al., 2010). Bells Palsy is a condition causing drooping of one side of the face.People often remember exposure to a cold before developing Bells palsy. Anywhere from 1 in 1000 to 1 in 250 people develops Bells palsy in any given year,most commonly striking people over 70 years . but could occur at any stage of life (Rister, 2003 ). Named after Scottish anatomist Charles Bell, who first described it, Bells palsy is the most common acute mononeuropathy (disease involving only one nerve) and is the most common cause of acute facial nerve paralysis (Salinas et al., 2010). Nutritional Supplements required for Bells Palsy Treatment , include: Vitamin B12 essential fatty acid(5,000 milligrams daily) Pyruvate(3000 milligrams daily) Vitamin E (400 IU daily) ( Rister , 2003). No treatment for Bells Palsy, whether conventional or alternative is more effective than vitamin B12, but if patient has shingles three other Supplements may also help to accelerate recovery. Laboratory experiments have shown vitamin E, Sodium Pyruvate (a support supplement) and membrane stabilizing free fatty acid (Rister, 2003). This project was designed to find out the biochemical basis of the recommendation of the pigeon meat by most of the physicians for the patients of Bells Palsy In the present project, the flesh of wild and domestic pigeons will be analysed and compared for the concentrations of oil , Tocopherol and Fatty acid profile. This comparative study will also furnish the scientific reason of preferable use of wild or domestic pigeon for the treatment of patients of BellsPalsy. The main objectives of the present study are as follows : To evaluate compare oil contents in flesh of wild and domestic pigeons To evaluate compare Tocopherol contents in flesh of wild and domestic pigeons To evaluate compare Fatty acid profile in flesh of wild and domestic pigeons REVIEW OF LITERATURE Pomianowski and Mikulski (2009) excised Two muscles, breast and thigh, from 3 pigeon meat-type breeds (Europigeon,WrocÅ‚awski, King) and were analyzed for proximate analysis, cholesterol content, and fatty acid (FA) profile. Among the breeds considered, the lowest protein content of breast muscle was found in Kings (21.73%), whereas the highest fat (7.07%) and ash (1.11%) content of breast muscle (P à ¢Ã¢â‚¬ °Ã‚ ¤ 0.01) was located in WrocÅ‚awski pigeons. The cholesterol content of both muscles was lowest in Europigeon (23.6 to 25.2 mg/100 g of tissue) as compared with the King and WrocÅ‚awski pigeons (30.2 to 44.4 mg/100 g of tissue). The total content of polyunsaturated FA was lower in thigh than in breast muscles (by 4.5 to 12%, depending on the breed). As far as FA composition is concerned, pigeon meat resembles meat types of poultry species. However, due to low cholesterol and a fairly high protein content, pigeon meat can be used as a valuable inc lusive component of the human diet. Young and Stagsted (2003) illustrated that in nonstressed birds, antioxidative status was reflected in decreased TBA-reactive substances (TBARS) in pectoralis major (PM ) ,iliotibialis (IL) and liver of ascorbic acid-ÃŽÂ ±-tocopherol-supplemented chickens and likewise in liver from oregano-supplemented chickens compared to that of nonstressed control birds. However, postmortem temperature, pH, and water-holding capacity were not affected by supplementation. Drip loss from oregano-supplemented chickens showed increased protein oxidation in specific bands, but this did not relate to water-holding capacity or antioxidative status. When exposed to stress, the concentration of TBARS in the control animals increased in PM and IL. Ascorbic acid-ÃŽÂ ±- tocopherol supplementation protected IL, and oregano supplementation protected PM from stress-induced increases in TBARS. This differential effect between muscles may indicate differences in protection mechanisms. In conclusion, ascorbic acid-ÃŽÂ ±-tocopherol and oregano supplements to chickens protect against stress-induced increase in TBARS, in different muscles. Abulude et al. 2006 determined the Anatomical weight, proximate composition, selected mineral contents and sensory evaluation in male and female pigeon birds ( Columba guinea G) found in Akure, Nigeria using standard methods . The species analyzed individually , were high in protein (60.63-66.92%), fat (13.28-15.34%) and ash (11.17-15.54%). Moisture, fibre, and carbohydrate contents varied within a narrow range. The mean energy level was 386.93  ± 30.45 Kcal. The mineral contents were highly variable. The abundant elements were Na, K, Ca and Mg and the remaining elements were present in amounts below toxic levels, while Cd was not detected. The sensory scores in terms of juiciness, attractiveness, tenderness, texture and flavor revealed that these samples were good for consumption. The chemical values indicate good quality protein and dietary minerals. Sheldon et al .2007 studied , the effect of varying dietary vitamin E levels on the oxidative stability, flavor, color, and volatile profiles of refrigerated and frozen turkey breast meat was examined. Breast meat was excised from four carcasses per treatment and evaluated after refrigeration (1 and 7 d) or frozen storage (30, 90,150 d) for oxidative stability and sensory quality by Thiobarbituric acid (TBA) analysis, descriptive flavor profiling, and headspace gas chromatography. The TBA values were inversely related to the dietary vitamin E levels. Refrigerated samples had TBA values 78 to 88% lower for the 10 ´ and 25 ´ vitamin E treatments, respectively, than for the NRC control treatment. No differences in TBA values (refrigerated samples) were detected for the 10 ´, 25 ´, and 20 ´ (3 wk feeding duration) or across all treatments for samples frozen for 5 mo. The 10 ´ and 25 ´ NRC diets produced the most typical and acceptable turkey meat flavors with the fewest oxidized off-flavor notes for both fresh and frozen samples as opposed to the more oxidized flavor notes detected in the control samples. Mean color scores increased, indicative of less pale meat, as the level and duration of feeding dietary vitamin E increased. These findings showed that varying dietary vitamin E levels significantly influenced the oxid ative stability and functionality of turkey breast meat. Dsemeth and Messeyne (1981), studied that the fatty acid pattern of the triglyceroides (TG) and phospholipids(PHL) from liver, adipose tissue and crop of the pigeon at various stages of posthatching development and determined the influence of the changing diet. In each tissue and at all ages PHL contained more stearic and polyenoic but less monoenoic acids than the corresponding TG. Especially in the young squabs the acid composition of the liver (TG as well as PHL) is different from that of the adipose tissue and the crop. In each tissue and at all ages of pigeons, the TG had a relatively simple fatty acid pattern consisting mainly of Ci6 and C, »components and containing more palmitic (16:0), palmitoleic (16:1) and oleic (18:1) acid but only trace amounts of long-chain polyunsaturated acids compared to the corresponding PHL. This class contained a higher amount of stearic (18:0) and linoleic (18:2) acid and up to 31% (liver at hatching) C20 and C22 polyenoic acids, mainly ar achidonic (20:4) and docosahexaenoic (22:6). MATERIAL AND METHODS Place of working : The study will be conducted at following laboratories : Research Laboratory , Department of Zoology, GC University Faisalabad Pesticide Chemistry Lab, Plant Protection Division ,Nuclear Institute For Agriculture And Biology (NIAB) . Specimen Collection : Random samples of domestic pigeon (Columba livia domestica) and wild pigeons (Columba livia livia ) will be procured from local supplier at Faisalabad city. Preparation Of Samples: The selected pigeons will be slaughtered ,defeathered and excised to take out four body parts ( Chest, Wings, legs liver ) . Proximate Analysis: Proximate analysis of the samples for moisture , Protein ,Fat , ash and minerals will be done according to the Association of Official Analytical Chemists; AOAC(2000) . Analysis For Tocopherol Fatty Acid For the analysis of Tocopherol Fatty acids composition, following methodology will be adopted : Blending / mixing of Flesh: High Speed blender ( Braun Multimix MX_32 , Germany ) will be used to mix flesh thoroughly . Extraction Of Fat From Pigeon Flesh: The extraction of fat will be done by using Soxhlet apparatus .25g of each sample will be taken in extraction thimbles .And 125 ml of n_hexane will be taken in 250ml round bottom flask (Quickfit).The extraction will be proceeded for 12 cycles to collect complete oil . Concentration of oil : From the extract of n_hexane and oil , n_hexane will be evaporated by using rotary evaporation apparatus . The extract will be transferred to Round bottom flask, and then will be evaporated the content of n-hexane at appropriate temperature of water bath (60 °c) under suction up to dryness. Preparation Of Fatty Acid Methyl Esters (FAME)- Fatty Acid Analysis -: Preparation of Fatty Acid Methyl Esters (FAME) and Fatty acid analysis will be done by using Official Method and recommended practices of American Oil Chemists Society ;AOCS(1993) . Tocopherol Analysis : For the qualitative and quantitative analysis of tocopherol HPLC coupled with Fluorescence detector will be used (Gliszczynsha Swiglo and Sikorsha ,2004) .Analysis of Tocopherol will be done by using all HPLC analysis of tocopherols at room temperature on waters 600 high performance liquid chromatograph(Waters , Millford ,M.A,USA). For determination of tocopherols in oils , a mobile phase consisting of 50 % acetonitrile (Solvent A ) And 50 % of methanol (Solvent B ) was used with the flow rate 1ml / min. Injection volume was 20  µl ; rheodyne injection was used . Tocopherols were identified by using their retention times with those of corresponding standards and by spiking of samples with appropriate standards .

Friday, January 17, 2020

Home Deferring Dreams in a Raisin in the Sun

In Lorraine Hansberry’s play, â€Å"A Raisin in the Sun,† she does a great job of intertwining Langston Hughes’ poem â€Å"Montage of a Dream Deferred,† to incorporate her underlying theme of dreams. In his poem, Hughes asks â€Å"What happens to a dream deferred? † and then goes on to list the different things that might happen to a person if his dreams are put â€Å"on hold. † His overall point is that whatever happens to a postponed dream is never positive. Meanwhile, the question Hansberry poses in her play is, â€Å"What happens to a person whose dreams grow more and more passionate — while his hopes of ever achieving those dreams grow dimmer each day? Dreams get put on hold for many different reasons but in the case of the Youngers, it was their home environment that ensured that none of them would be able to accomplish their ultimate dreams. Lena, Walter, Ruth, and Beneatha Younger were a poor African American family that share d a small one-bedroom apartment in the south side of Chicago. Each person had vastly different goals and dreams. Being the head of the household, Lena dreamed the dreams of her children and would do whatever it took to make those dreams come true.Walter, Lena's oldest son, set his dream on starting his own business with a liquor store. He had the basic â€Å"American Dream† of starting from the bottom before ultimately working your way to the top with his entrepreneurial spirit. Beneatha, on the other hand, wanted to become a doctor when she got out of college and Ruth, Walter's wife, wanted to be wealthy. While trying to reach these dreams, each member of the the Younger family had their own dreams postponed and put on hold at some point or another for various reasons.Lena was a widow in her early sixties who devoted her life to her children after her husband's death. Retired from working for the Holiday's family, she was waiting for her husband's insurance money to arrive. With the ten thousand dollar check in her hand, Lena decided to buy a three thousand dollar house in Clybourne Park and she was also going to put some of the money in the bank for Beneatha's medical school. She realized this money was a one-way ticket for her family to get out of their environment and improve their lives and believed buying a house in a different neighborhood was the best way to do this.However, Walter was upset when he heard his mother had spent the insurance money on the house and thought it wasn't fair that Beneatha got some of it for her medical school while he got nothing for his liquor store business. Lena, who always wanted her son to be happy, trustingly gave the rest of the insurance money to Walter. However, he then gave the money to Bobo and Willy, two of his friends with questionable character, to help him get his liquor license. Unsurprisingly, Willy betrayed Walter, taking off with the money and causing his dream to crumble to pieces.Walter was deceive d by his friend Willy but the reality is his dream was never going to happen anyway, and the rest of the family knew this. Living where they lived, the environmental pressures were extremely high. There were five people living in a tiny, run-down, roach-infested one-bedroom apartment, with two families sharing a bathroom. Everyone was looking for a way to improve their lives and Walter wanted to be the one to do it with his liquor store. â€Å"Sometimes it’s like I can see the future stretched out in front of me – just plain as day. The future, Mama.Hanging over there at the edge of my days. Just waiting for me – a big, looming blank space – full of nothing. Just waiting for me† (980). Walter knew there was no future ahead of him if he continued on his life path and he knew he needed to get out. Living in this type of environment, your dreams will always be put on hold until you can finally get out. Ruth, Walter's wife, was pregnant at the time her husband was trying to start up his liquor store and she realized her dream of being wealthy and having a fine family was simply just that – a dream.To her, it was a consolation that her husband had come back to reality after his goals fell through. The problem Walter faced and the reason he was so unsuccessful was that his main goal was not to escape their environment, but merely to improve it. Due to where they lived, the family was destined to fail unless they made a move to get away from it. A lot of the family realized this but Walter didn’t. As Kristin Mathews says in her article â€Å"The Politics of â€Å"Home† in Lorraine Hansberry’s A Raisin in the Sun, â€Å"Walter’s failing is his acceptance of the capitalist economic system that necessarily excludes him from ascendancy† (par. ). She says that Walter doesn’t wish to change the system but merely change his position in it and until the very end of the play, he is willing to â€Å"surrender his will to this system and exchange his dignity for whatever ‘life’ it might offer him and his family† (7). Unlike Walter, Beneatha, on the other hand, knew that she wanted to escape the system altogether. This is why she was extremely upset when she found out Walter didn't put anything in the bank for her medical school because she knew that was her way out.She gave up hope and her dream of becoming a doctor seemed to fade away with Walter’s liquor store business. Fortunately, her friend Asagai came over and took her out of her environment. By marrying him and moving to Nigeria to practice her medical career, she found her new ticket out of the environment and system that was setting her up for failure. Lena was also well aware of the difficulties of living where they did. She knew moving away was the best decision for the family which is why she bought the house in Clybourne Park with some of the insurance money she received.However, pr ior to the family moving, Mr. Lindner, a representative from Clybourne Park, offered to pay the Youngers to not go into his neighborhood. Lindner, along with the rest of the community, didn’t want a black family living in their neighborhood. Taking the money would have been immoral in the family's eyes, and prioritizes money over human dignity. They understand that moving is the best choice for the family but once again, Walter did not. He was willing to push all his ethical beliefs to the side to take the money and improve his life within his system before ultimately changing his mind.Even though the road ahead will be difficult, they know that they have made an honorable choice and have finally gotten out of the environment that has been holding them back this whole time. They didn’t just improve their lives within the system, they got out of it. Lorraine Hansberry had successfully described the four main characters in the story as human beings with desires, dreams, aspirations, conflict, foibles, and strength. It was â€Å"A Raisin in the Sun† that expressed those dreams and desires and how they ended up as â€Å"dreams deferred. Once the family was finally able to leave their home environment in the south side of Chicago, their dreams began to form into more of a reality. A major underlying theme of the book is to not give up on your dreams and do whatever it takes to accomplish those dreams as soon as possible. It is very rare that putting your dreams on hold turn out in a positive way so you need to seize the moment at all times and push aside anything that holds you back. The Youngers realized that what was holding them back was the system of their home environment and they got away from it as soon as they could and they were happier for it.Works Cited Hansberry, Lorraine. â€Å"A Raisin in the Sun. † The Norton Introduction to Literature. By Alison Booth and Kelly J. Mays. New York: W. W. Norton &, 2011. 950-1021. Print. Hu ghes, Langston. â€Å"What Happens to a Dream Deferred? † The Norton Introduction to Literature. By Alison Booth and Kelly J. Mays. New York: W. W. Norton &, 2011. 950. Print. Kristin L. Matthews. â€Å"The Politics of â€Å"Home† in Lorraine Hansberry’s A Raisin in the Sun. † Modern Drama 51. 4 (2008): 556-578. Project MUSE. Web. 14 Mar. 2013. .

Thursday, January 9, 2020

Humans s Views On The Evolution Of The World And The Life...

Americans Resistance to the Fact of Evolution Michael Nelson Dr. Baldwin REL2121 3 / 22 / 2016 THESIS: Humans who believe in creationism are usually resistant to the views of scientists on the development of the world and the life that inhabits it, but their views continue to be challenged by the empirical evidence of biology and science. This bringing attention to the question, will resistance toward evolution ever let up? OUTLINE: I. Introduction II. Creationism A. Definition of Creationism 1. Opposed to evolution a. Charles Darwin 2. Protection of Beliefs b. Curriculum III. Evolution A. Definition of Evolution 1. Facts B. Resistance 1. Why so many American’s are resistant to evolution†¦show more content†¦They want to believe that a higher being has a meaningful purpose for each of us, and we were created by something bigger than us, a God. Therefore they are resistant to evolution and create theories to make their view more realistic, for example, intelligent design. Intelligent design is a view that the creation of living things is best explained by an intelligent cause, basically the opposite of natural selection. The scientific community continues to say intelligent design is a form of creationism that lacks empirical support and offers no testable hypotheses. The continuous discoveries in biology and science continue to provide more and more empirical evidence to prove that evolution is real. So why are Americans so resistant to the evidence and scientific proof of evolution, and will creationists ever begin to accept the proven facts that invalidate everything they think is tr ue about creationism? Creationism is a view that less than fifty percent of Americans believe in. According to an article on Stanford’s website, a creationist is someone who believes in a god who is the absolute creator of everything that inhabits the earth.1 Creationists strongly oppose a world brought on by evolution, particularly a world described by Charles Darwin in his book On the Origin of Species that was written in 1859. Darwin’s book introduced the scientific theory that we evolve over generations

Wednesday, January 1, 2020

Belief Systems in Macbeth - 1619 Words

Belief systems play an important role in the lives of humans as they govern a person’s thoughts, words and actions and often reflect the predominant values of a specific period in time. In Shakespeare’s play Macbeth, three different belief systems are present: belief of the Supernatural, the Great Chain of Being and Divine Justice. Belief in the Supernatural is the belief that factors such as fate, astrology and nature determine the path of a person’s life and may become self-fulfilling prophesies. The Great Chain of Being is the belief in hierarchical systems that once disrupted will result in chaos. Lastly, Divine Justice is the belief that all actions will be reciprocated, either though punishment or reward. Each of these systems share†¦show more content†¦Her guilt is evident when she is sleepwalking and says, â€Å"Here’s the smell of the blood still. All\ the perfumes of Arabia will not sweeten this little\ hand.† (5.1.50-52) Shortly after, during the attack on Macbeth to claim the throne for Malcolm, Macbeth hears a woman’s scream and questions, â€Å"Wherefore was that cry?†, Seyton responds, â€Å"The Queen, my lord, is dead.† (5.5.15-16) Lady Macbeth kills King Duncan, and this reveals that while she does capture the throne, her days as the sovereign queen are short-lived. The disruption continues when Macbeth follows through with his wife’s plans and kills the King of Scotland. He says to Lady Macbeth, â€Å"I have done the deed.† (2.2.15) Unbeknownst to Macbeth, the witches placed a spell on him preventing him from being able to sleep if he killed the king. Sleep shall neither night nor day Hang upon his penthouse lid. He shall live a man forbid. Weary sev’nnights nine times nine Shall he dwindle, peak and pine. (1.3.19-23) Unable to sleep, Macbeth is unable to function properly, and starts to see the ghost of Banquo, who he had just had murdered by assassins. Avaunt, and quit my sight! Let the earth hide thee! Thy bones are marrowless, thy blood is cold; Thou hast no speculation in those eyes Which thou dost glare with! (3.4.94-97) This suggests that Macbeth is feeling guilty for killing his friend and suffers mentally as a result. The disruption ofShow MoreRelatedAnalysis Of Grendel And Macbeth 915 Words   |  4 Pagesand sadness† (Herrera). In Grendel, tragedy and confusion is seen throughout the story. The theme in the story of Macbeth is tragedy and pain. This early literature exemplifies evil through these themes. The philosophers whose theories support these themes through British literature are Jean-Jacques Rousseau, Sigmund Freud and Fred Alford. Throughout the stories of Grendel and Macbeth, evil is displayed differently as well as the theories that contribute to the evil seen. 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